1. Looks like most of them are
Campylobacter jejuni which is followed by
C. coli, and there are rare of
C. fetus. I may have killed all of
C. fetus "unconciously" (when I raised the temperature up to 42C).
2. The high concentration of primers cause primer dimers.
3. The 23S bands don't show in each well => may not correlate with Campy very well
4. A few of "suspected Campys" are actually not campys. Although they grow on selective agar and in microaerobic condition, they're unlikely to be Campys from genetics viewpoint. (no wonder I felt they grow unusually faster than they're supposed to be)
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