Wednesday, November 02, 2005

mPCR and reagents

To do multiplex PCR, many chemicals have to be prepared. Also, I was told by Ray that it'd be better to purchase my own reagents. That is... Taq (for hot-start) Taq buffer dNTP mix primers TAE/TBE (electrophoresis buffer) 200 ul PCR tubes Bromophenol blue (dye) Ethidium Bromide/SYBR Green DNA marker reference cultures (positive/negative controls) ...Huuhh...Even though Eppendorf company would be cheaper to purchase from, that must be still a big expense...:( How'd Peter feel...

No comments: