Wednesday, November 02, 2005
mPCR and reagents
To do multiplex PCR, many chemicals have to be prepared. Also, I was told by Ray that it'd be better to purchase my own reagents. That is...
Taq (for hot-start)
Taq buffer
dNTP mix
primers
TAE/TBE (electrophoresis buffer)
200 ul PCR tubes
Bromophenol blue (dye)
Ethidium Bromide/SYBR Green
DNA marker
reference cultures (positive/negative controls)
...Huuhh...Even though Eppendorf company would be cheaper to purchase from, that must be still a big expense...:( How'd Peter feel...
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