Friday, December 09, 2005
Agar dilution and microdilution test on Campy
Campy samples: Campy from yesterday's broth inocula (overnight incubation, in 3ml Muller-Hinton broth). Camp120-125 (except 122 - initial inoculum too few); ie 5 isolates.
1.Agar dilution:
Agar: Campylobacter Selective Agar ("CSA"; charcoal agar) w/o antibiotics
Honey final conc: 0%, 2.5%, 5%, 7.5%
Inocula conc: 10^3, 10^6, 10^9
Temp: 37C and 42C
2. Microdilution
Dilution broth: Muller-Hinton broth
Honey conc: 15% honey serial dil'n
Inocula conc: 10^6
Temp: 37C
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