Campy mPCR (x46 isolates...!)

1. Looks like most of them are Campylobacter jejuni which is followed by C. coli, and there are rare of C. fetus. I may have killed all of C. fetus "unconciously" (when I raised the temperature up to 42C). 2. The high concentration of primers cause primer dimers. 3. The 23S bands don't show in each well => may not correlate with Campy very well 4. A few of "suspected Campys" are actually not campys. Although they grow on selective agar and in microaerobic condition, they're unlikely to be Campys from genetics viewpoint. (no wonder I felt they grow unusually faster than they're supposed to be)