Discussion with Ray (and David...?)

As I've got some strange result in time-effect test when comparing with Enterococcus faecalis and Pseudomonas aeruginosa, I took those results down to Ray's place and had a small discussion. David was there as well. It looks like this result interested Ray and David, and they even thought this has to do with resistant gene and would like me to screen there genes. Not quite sure what resistnat genes may be responsible to this, Ray asked David which gene should be screened. Guess what David answered - 16S?! (Even Ray was surprised... :P ) Ray pointed out that the volume I'd been using (20ul) is too small and, I should use 100ul-1 ml instead. He also considered that the inoculum density of mine (100,000 CFU/ml) is too heavy and may reduce it down to thousands or even hundreds of CFU/ml. However, 20ul is what we've been using in our lab, and also 100,000 CFU/ml is the standard in CLSI (previously called NCCLS). Dillema...