My helicobacter looks still contaminated by other cutlures; from the gram's stain, many gram positive cocci can be seen (I guess enterococci). Now I'm using urea agar (columbia agar + serum + HCl + urea + phenol red) and trying to get single colony for recovering helicobacter, but honestly I don't expect too much on it now... :(
Ray told me that I could add vancomycin to get rid of enterococci. Actually this has been included in the urea agar plates, so give them a few more days and see what'll happen. If these stock cultures are still unrecoverable, I reckon I'll need to re-order another copy of helicobacter type strain from ESR.
In the photo Ray said the faint pink organisms (the gram negative ones) should be helicobacters but I doubt it; they look too fat as helicobacter.
Thursday, September 28, 2006
Helicobacter contamination (still)
My helicobacter looks still contaminated by other cutlures; from the gram's stain, many gram positive cocci can be seen (I guess enterococci). Now I'm using urea agar (columbia agar + serum + HCl + urea + phenol red) and trying to get single colony for recovering helicobacter, but honestly I don't expect too much on it now... :(
Ray told me that I could add vancomycin to get rid of enterococci. Actually this has been included in the urea agar plates, so give them a few more days and see what'll happen. If these stock cultures are still unrecoverable, I reckon I'll need to re-order another copy of helicobacter type strain from ESR.
In the photo Ray said the faint pink organisms (the gram negative ones) should be helicobacters but I doubt it; they look too fat as helicobacter.
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