Thursday, September 22, 2005

Campylobacter (and some others)

It seems like not many researches are done about Campylobacter due to its difficulty of culturization. Even if they do, there isn't too much information about the sensitivity of Campylobacter on honey. Since this is a multi-resistant bacteria, I think it is worth to try to examine its MIC thoroughly. Professor as well says that Campylobacter is in fact one of the most important entero-pathogens in NZ. Hence this may be my first material in near future. As a pre-test, I'm going to pick this difficult-culture up tomorrow. It'll be inoculated on CSM (Campylobacter Selective Media) which is in fact a kind of charcoal agar, and it is literally black (due to the charcoal). It looks yuck! Its stock cultures, unlike other normal bactria, should be stored in -70 ºC freezer. Its incubation also has to be carried out not in the normal incubator but in the CO2 one (also the gas condition may need some more modifications). It's somewhat troublesome. On the other hand, I found there are some "minor" bacteria classified into Enterobacteriacea family yet not be reported too often. For example, an opportunistic organism Kluyvera genus (Kluyvera ascorbata sp. and Kluyvera cryocrescens sp.) produce β-lactamase which also means they would resist to β-lactam antibiotics such as penicillins and cephalothins. Would it be worth to examine these kinds of minor bacteria as well? Maybe some more literature reviews are required.

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