DNA extraction

To do PCR, DNA extraction is unavoidable, and thus I have to extract that of Campylobacter isolates. As I'm not using these DNA for SEQUENCING or CLONING but just differentiate what species they are, I wouldn't spend too much time on extraction and purification, hence I choose one of the simplest ways - boiling method - to do my work today. (Lysis method requires some more chemicals and that would increase my expense) 1. Boil Campys Normally I have to boil bacteria 10 min and suddenly chill on ice for another 10 min, but unfortunately the lids of eppendorf tubes pop out as the air heated up and I tried hardly to tie them up with celotapes (don't work) and sterilize tapes (more or less work) 2. Chloroform purification Unfortunately our chloroform has run out at the moment and Kerry borrowed a small bottle of chloroform (~80mL) from another lab. As this is a corrosive material, I'll have to record how much I use and write down on a track sheet, and collect the waste into another empty 2.5L winchester bottle. Eventually I used 2.8mL. Only 2.8mL chloroform in a big 2.5L winchester bottle... Looks stupid... -.-;; 3. Add NaOAc and Ethanol precipitate DNA Here's really a problem. I've got 1mL of ethanol and DNA sample in each eppendorf tube and have to centrifuge 5min - with the horizontal centrifuge in our lab. Oddly, the lid pop out and my samples almost flow out! Concerning my sample, I asked Kerry to change the roter into a "normal" one and, for in case, I tried to centrifuge some tubes with water to see if it's there's any problem. But alas! After testing 5 min centrifuge with water, the water gets warm and the roter is shockingly hot - actually it's untouchable! What would happen if ethanol is heated in capped tubes? (you can imagine how dangerous it is). This centrifuge is said to be around 15 years old... As this is unusable, I'd have to go down to Ray's lab and centrifuge my samples there... 4. Ethanol precipitation not needed?! In Ray's place, he told me I just need to use chloroform and then run pcr - ethanol not needed! This really surprised shocked me. Now I see his protocols are somehow different from normal ones...