Multiplex PCR

I've done PCR before (Er...I mean 4-5 years ago), but this is my first time to do multiplex one. Actually their principles are quite similar - except the number of primer pairs). The result shows that of 14 Campylobacter isolates (plus 1 negative control), most of them are C. jejuni which is followed by C. fetus and then C. coli; no other species (such as C. upsaliensis and C. lari). # It looks like the samples from Medlab are not necessarily pure cultures... :( # Method n Material: DNA extraction: boiling method with chloroform purification Taq: Eppendorf HotMaster Mix Thermocycler: MJR PTC-100 Electrophoresis system: ????? Electrophoresis gel: agarose dissolve in SB buffer (with Ethidium Bromide dissolved) Electrophoresis buffer: SB buffer (not TAE or TBE buffer!?) Gel imaging system: ??????